negative effect of heat sterilization on the free amino acid concentrations in infant formula - glass water bottles for office
Infant formula milk powder is usually hot disinfected in hospitals, hospital infection or water pollution is a problem, but few studies have shown the effect of high temperature on the nutritional value of infant formula milk powder.
In particular, the effect of heat sterilization on free amino acids (FAA)
Concentration is rarely discussed.
Given the importance of these nutrients to the growth of infants, we investigated the FAA concentration of infant formula milk powder after heat sterilization.
Reformulation of infant formula milk powder with hot water (80°C)
In the glass bottle, put 5 min in the autoclave under pressure of 105 °c and 5600 kg/m2.
Additional formula samples were prepared as controls by conventional methods.
After high pressure sterilization, we measured the FAA concentration by ion exchange chromatography.
The results were compared with those after conventional preparations. We found a 19.
Compared with traditional preparations, the quality of total protein decreased by 5% after high pressure sterilization.
After high pressure sterilization, the concentration of total FAA was significantly reduced (696. 5±101. 4 vs 899. 4±152. 2u2009μmol/l, P=0. 01).
The concentration of individual amino acids in the high-pressure sterilized infant formula is also low, with the difference between-4. 1 to 71. 5% (mean 22. 6%).
The concentration of certain amino acids is reduced by more than 30%, such as valine (71. 5%), citrulline (61. 1%), glutamine (60. 6%)Ethanol (54%), and lysine (39. 2%).
Both essential and non-essential amino acids are affected by high pressure sterilization, 28. 17 and 27.
13% lower than the control (P=0. 37).
The concentration of ammonia increased significantly after high pressure sterilization (645. 2±76. 2 vs 393. 2±140. 7u2009μmol/l, P=0. 0003).
However, the urea level after high pressure sterilization is significantly lower than that after conventional preparation (1110. 8±162. 7 vs 1426. 5±209. 5u2009μmol/l, P=0. 0004).
The accumulation of ammonia may reflect the degradation of protein and amino acids.
High pressure sterilization significantly led to a decrease in FAA concentration in infant formula milk powder.
Whether the increase in ammonia concentration after high pressure sterilization will lead to harmful effects is worrying. Although breast
Feeding is considered superior to formula feeding for normal infants, and many babies still receive formula from birth.
Infant formula milk powder is usually heated and disinfected in hospitals where water pollution or hospital infection is a problem, especially in developing countries, when nurseries and hospitalized babies need to prepare milk in large quantities every day.
However, only a few studies investigated the effect of heat on the nutritional value of infant formula (; ; ).
High temperatures have been shown to result in a significant loss of several vitamins, including thiamine, vitamin B6, ascor, folic acid, and vitamin b12.
The dehydration form of asa produced when heating milk is involved in the oxidation reaction affecting folic acid and vitamin B12 (; ).
However, other researchers believe that, in addition to thermal damage, heat treatment has no serious harmful effect on nutritional value
Unstable vitamins that are easy to supplement (; ).
What is lacking is the study of the effect of heat sterilization on free amino acids (FAA)
The content of infant formula milk powder.
In view of the importance of inf for infant nutrition and growth, and the FAA content partially reflecting protein quality, we designed this study to investigate the quantitative changes in protein and FAA content in high-pressure sterilized infant formula milk powder.